For this work, in vivo, in vitro, metagenomic, and metaproteomic studies were conducted with an anaerobic microbial community derived from sewage sludge that quantitatively produced toluene from phenylacetic acid (via decarboxylation). (jbei.org)
A team from the U.S. Department of Energy's Joint BioEnergy Institute (JBEI) and the Lawrence Berkeley National Laboratory conducted an intensive study of the toluene - producing enzyme PhdB — a glycyl radical enzyme (GRE) of bacterial origin that catalyzes phenylacetate decarboxylation, and its cognate activating enzyme PhdA, a radical S - adenosylmethionine enzyme — discovered in two distinct anoxic microbial communities that produce toluene. (rdmag.com)
Surprisingly, none of the candidate mechanisms previously implicated in hypothalamic leucine sensing (KATP channels, mTORC1 signaling, amino - acid decarboxylation) were involved in the acute activity changes produced by l - leucine. (molecularmetabolism.com)