B and C, HuH - 7 cells were cotransfected with a p21waf1 / cip1 promoter — driven reporter (p21 - Luc) or its derivatives represented in (A)(0.25 μg of each) together with DDX3 expression construct (pcDNA3 - SRα / FLAG - DDX 3) or control vector (pcDNA3 - SRα / FLAG; 2 μg). (cancerres.aacrjournals.org)
To this end, increasing amounts of expression constructs of DDX3 / DQAD, DDX3 / AAA and wild - type DDX3 were cotransfected with p21waf1 / cip1 promoter — driven luciferase reporter. (cancerres.aacrjournals.org)
The results suggest that selecting expression constructs for crystal trials based primarily on expression solubility is insufficient. (jcsg.org)