This chemical is also found in the dog flea medication Program and is primarily designed to disrupt the reproductive capabilities of female adult fleas. (mypetneedsthat.com)
The test detects adult heartworms if there are more than 5 female adult heartworms present (the test doesn't recognize a pure male heartworm population). (dogsnaturallymagazine.com)
Here is a summary: Large - scale commercial dog breeding kennels are defined as facilities housing more than 15 intact female adult dogs for breeding. (nationalpuppymillproject.org)