Total RNA was reverse transcribed with RT2 First Strand Kit (SABiosciences) according to the manufacturer's instructions. (journals.plos.org)
cDNA was synthesized using the RevertAid H Minus First Strand cDNA synthesis kit (Fermentas), using both random hexamer primers and oligo - dT primers. (genetics.org)
Subsequently, equal amounts of total RNA (300 - 500 ng) were reverse transcribed into cDNA using random primers (SuperScript First Strand Synthesis for RT - PCR; Invitrogen, Carlsbad, CA). (molvis.org)