After three weeks, all medium from the six - well cultures was removed, followed by a single media wash and replacement with fresh medium for 24 hours. (journals.plos.org)
A benefit of the multiple harvest strategy is that each day you are supplying your producer cells with fresh medium. (blog.addgene.org)
300,000 cells were plated per well of a 24 - well plate (minimum 10 wells) and mixed with virus (80 ng p24 / 106 cells) diluted in 100 µl of fresh medium. (journals.plos.org)