Dilutions of the purified and pooled PCR amplicons were made and quantified using a synthetic oligonucleotide standard of known molarity [35], to approximate an optimal bead to template ratio for emulsion PCR. (investigativegenetics.biomedcentral.com)
Protein samples were diluted to the linear range of the assay, the weight of protein / ml was calculated in triplicate and the molarity was calculated using the known weights of the proteins. (journals.plos.org)