The phrase "opposite orientation" refers to something that is going in the complete opposite direction or facing the opposite way. It implies that two objects or ideas are positioned or aligned in a contrasting or different manner. Full definition
The construct contains two tandem invertible segments, each with two fluorescent protein coding sequences in opposite orientations. (blog.addgene.org)
The first patent on «active noise cancellation» dates to 1933, when German physicist Paul Lueg proposed to silence sound waves by simultaneously generating waves of the exact opposite orientation. (discovermagazine.com)
The black and white spots represent magnetic field concentrations with opposite orientations, called polarity. (solarviews.com)