In addition, PLXDC2 was identified as a BACE1 substrate in primary mouse neurons in two independent studies (Kuhn et al, 2012; Zhou et al, 2012). (life-science-alliance.org)
The following primary mouse monoclonal antibodies were used: MITF (1 ∶ 30, Abeam), RLBP1 (CRALBP, 1 ∶ 1000, Affinity Bio reagents); BEST1 (1 ∶ 1000, Millipore); RPE65 (1 ∶ 500, Millipore); PMEL17 (1 ∶ 100 Dako); KRT8 (1 ∶ 2000, Millipore); Na + / K+ATP ase (ATP1B1 - 1 ∶ 100, Abcam). (journals.plos.org)
Latex bead labelling and comparative immunofluorescence analysis of the p62 subunit of the TFIIH was performed as described previously [16,17] using primary mouse embryonic fibroblasts at passages 2 — 5. (journals.plos.org)