Automated and manual annotation to produce the GENCODE reference gene set (v7) of protein - coding genes, pseudogenes, and non-coding RNAs. (massgenomics.org)
REST calculates the relative expression ratios on the basis of group means for target genes versus reference genes, and tests the group ratio results for significance, comparing normalized and not - normalized expression results. (journals.plos.org)
Cq values of target genes of all samples were normalized to a B. burgdorferi reference gene (16S rRNA) or mouse reference gene (β - actin) to compensate for sample - to - sample and run - to - run variations and to ensure experimental reliability. (journals.plos.org)