Future researchers could apply this technique to efficiently deliver large repair templates for applications that require large gene fusions. (blog.addgene.org)
If a user wants to carry out a high - throughput genetic screen, it is expensive and time consuming to individually generate gRNAs and homology arm repair templates containing the tag for each insertion. (blog.addgene.org)
In fact, the Mitalipov group found that cellular repair mechanisms, at least in their experiments, did not respond very well to the introduction of a synthetic repair template added by the researchers. (scientificamerican.com)