Otoferlin's size has precluded rescue experiments in which a modified mRNA for otoferlin is transfected into an animal model to replace a suppressed or knocked - down otoferlin gene causing deafness. (sciencedaily.com)
To further validate the essential role of MELK in BBC cells, we carried out rescue experiments with both WT and kinase inactive MELK. (elifesciences.org)
To further validate that the decreased metastasis induced by double knockdown of ABL1 and ABL2 was not due to off target effects of the lentivirus - encoded shRNAs, we used a second set of ABL shRNAs (shAA # 2) and carried out rescue experiments by expressing mouse Abl1 and Abl2 constructs (mAbl1 / Abl2) that are resistant to shRNAs against human ABL1 and ABL2. (stke.sciencemag.org)