Finally, DNA appeared on the scene, the ultimate holder of information copied from the genetic RNA molecules by reverse transcription. (religion.blogs.cnn.com)
cDNA synthesized by reverse transcription of 1 µg RNA primed with oligo (dT) and random 9 - mer primers was used as the substrate for quantitation of mRNA expression levels by quantitative RT — PCR in the presence of SYBR ® Green (Stratagene, La Jolla, CA). (molvis.org)
Quantitative real time reverse transcription polymerase chain reaction (real time qRT - PCR) was used to assess the expression of carcinoembryonic antigen (CEA) and mammaglobin mRNA in the peripheral blood of patients and healthy controls. (scirp.org)