The result was an extremely high - throughput sequencing protocol that, in a single run, can cover portions of the genomes of over 50,000 microbial cells (2). (sciencemag.org)
He took the samples back to the lab on dry ice, then ran them through the typical microbial DNA sequencing protocol: extract DNA from the samples, amplify microbe - specific «barcodes» known as 16S rRNA using a technique called polymerase chain reaction (PCR), run them through a sequencer and analyze the data. (sciencedaily.com)
The standard library amplification step in most sequencing protocols substitutes erroneous bases for the ones that have been modified, yielding high - quality but incorrect sequences. (sciencemag.org)