(C) In vitro expanded human Lin - CD127 + cells or (D) mouse Lin - CD127 + c - Kit + Thy1.2 + cells expanded from splenocytes of Rag1 - / - mice were treated with lethal toxin for 2 hr followed by IL - 23 stimulation for 30 mins. (journals.plos.org)
Once optimal detector gains were determined, an extensive brightness index of dyes was established on this instrument using sequential single staining of mouse splenocyte with 43 different antibody conjugates against mouse CD8a (53 - 5.8). (immunology2018.org)
Positive controls were CD4 + T cells isolated from animals prior sensitized with two immunizations of 3 × 107 donor splenocytes over 10 days. (journals.plos.org)