The advancement of the Cas9 - based platform for screening and validation will help further the development of new therapeutic products, and Caribou's CRISPR - Cas9 technology can utilize guide RNAs specific for unique sequences and target a gene at numerous sites and therefore provide enhanced specificity. (cariboubio.com)
Using targeted deep sequencing and unbiased whole - genome off - target analysis to assess Cas9 - mediated DNA cleavage in human cells, we demonstrate that «enhanced specificity» SpCas9 (eSpCas9) variants reduce off - target effects and maintain robust on - target cleavage. (science.sciencemag.org)
The CRISPR — Cas9 RNA - guided endonuclease system allows precise and efficient modification of complex genomes and is continuously developed to enhance specificity, alter targeting and add new functional moieties. (dynamic-biosensors.com)