Both 3DISCO and CLARITY have relied on the use of transgenic mice in which a subpopulation of neurons are brightly labeled with GFP (e.g. Thy1 - YFP - H mice). (blog.addgene.org)
For their investigation, the researchers first developed a disease model of transgenic mice expressing elevated levels of TDP - 43 to capture the pathology of sporadic ALS / FTD. (sciencedaily.com)
(B) GUS induction in transgenic plants in response to increasing ligand concentration. (journals.plos.org)