Cell viability was evaluated using trypan blue exclusion assay in triplicate. (cancerres.aacrjournals.org)
Cell viability and apoptosis following treatment of Karpas 299 and SU - DHL1 cells with increasing concentrations of rapamycin and mTOR silencing (siRNA) were assessed using trypan blue exclusion assay and Annexin V staining, respectively. (cancerres.aacrjournals.org)
Cells were incubated at 37 °C in 5 % CO2 for 20 h. External fluorescence was removed by treatment with trypan blue for 10 min. (journals.plos.org)