This forced movement of water changes vaginal cell shape and biology, leading to irritation, tissue damage and disruptions of the vaginal ecosystem. (fairhavenhealth.com)
For estrogen shots to be effective, they must be given after the dog has released eggs from the ovary (evidenced by concentration of progesterone in blood greater than 4 - 10 ng / ml) but before she goes out of heat (evidenced by assessment of vaginal cells on a swab). (lowchensaustralia.com)
This unnatural and forced movement of water changes vaginal cell shape and biology for the worse and can lead to tissue damage, irritation, and general disruptions to the vaginal ecosystem. (americanpregnancy.org)