My religion requires that I take three virgin females from the wild for ritual sacrifice. (religion.blogs.cnn.com)
In each scenario, virgin females transformed into nurturing moms in response to pup calls. (discovermagazine.com)
F1 virgin females were collected, transferred to individual cells of plastic 24 - well culture plates (various manufacturers) and given a single fly host to lay eggs. (journals.plos.org)