Using mass spectrometry - based analysis and bioinformatics comparisons, the team identified 889 proteins in conditioned HMEC medium and compared these proteins to those identified in whole cell lysates and the serum added to the cell growth medium. (pnnl.gov)
The antibody was incubated under agitation with Protein G beads for 10 min, HeLa DFO treated whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10 min under agitation. (abcam.com)
«Visualizing single molecules in whole cells with a new spin: A new imaging method could put super-resolution microscopy within reach of most biologists.» (sciencedaily.com)