Sentences with phrase «adherent cells»
"Adherent cells" refers to cells that attach and stick to a surface or substrate, such as the walls of a tissue culture dish. These cells need a surface for support and growth, and they cannot survive or multiply freely in a liquid medium. Full definition
(b) Colony - forming efficiency (CFE) of parental cells, hypoxia - exposed adherent cells (MDA - MB 231 A3 and BCM2 A3), and the cycling hypoxia - selected subpopulations (MDA - MB 231 F3 and BCM2 F3).
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The scientists then tried the proline / polyproline combination in a test system of adherent cell cultures that were subjected to freeze - thaw conditions.
Furthermore, we found that the stem - like and EMT phenotypes observed in the cycling hypoxia - selected subpopulation are not reversible, because we obtained the same molecular profile from this subpopulation by culturing them as spherical cultures for several months in normal oxygen content and culturing them as adherent cells (Figure S2 in Additional file 2).
The Core has the capability of producing large scale adherent cells cultured in either 850 cm» or 1750 cm» roller bottles grown on a roller apparatus in a 37 °C warm room.
D, the expression of miR - 7 was examined in adherent cells and mammospheres derived from MCF7 - BoM cells by TaqMan PCR.
Bone marrow - derived mesenchymal stem cells (BM - MSCs) are obtained by isolating mononucleated cells from a marrow aspirate by centrifugation on a density gradient, followed by adherent cell expansion in tissue culture.
Also, a power law as commonly found in the rheology of adherent cells does not capture cortical rheology as it is devoid of a characteristic timescale.
At the end of this incubation, all rafts were thoroughly washed with PBS to remove any non-adherent cells, while adherent cells were trypsinized and counted using a hemocytometer.
To determine whether the cycling hypoxia - selected subpopulations cycle slower than the parental and hypoxia - exposed adherent cells, equal numbers of cells from each cell population were plated on day 1, and an increase in cell numbers over 5 days was used to assess the rate of proliferation.
To compare the rate of proliferation under the same condition as the parental and hypoxia - exposed adherent cells, we plated the cycling hypoxia - selected subpopulation as adherent cells (adherent MDA - MB 231 F3 and adherent BCM2 F3) and measured the increase in cell numbers together with the other adherent cell populations.
While the parental and hypoxia - exposed adherent cells display similar rates of proliferation, the cycling hypoxia - selected subpopulations grow slower as adherent cells and even slower as suspension cultures (Figure 4c).
More than 90 % of the adherent cells were renal fibroblasts as identified by their elongated morphology and that they were positive for chicken anti — fibronectin (Abcam, Cambridge, MA) and mouse monoclonal anti-α-smooth muscle actin (Sigma - Aldrich) antibodies.
(c) Expression of EMT - associated proteins in the parental cells (P), hypoxia - exposed adherent cells (A3), and the cycling hypoxia - selected subpopulations (F3).
Mesenchymal stromal / stem cells (MSCs) are a heterogeneous population of plastic - adherent cells that exhibit a fibroblast - like morphology, generate colony - forming units (CFU - Fs) and can differentiate into osteoblasts, adipocytes, and chondrocytes, among others.
Adherent cells were washed once (PBS supplemented with 100 mg / L calcium chloride and 100 mg / L magnesium chloride), fixed (4 % paraformaldehyde, 10 min), then permeabilized (0.3 % Triton X-100 in PBS, 30 min).
After binding, cells were washed once (carefully using a 200 μl pipette to remove the medium) with RPMI with 0.5 % BSA solution and adherent cells were analyzed by immunofluorescence microscopy or bright field microscopy for quantification.