Sentences with phrase «axial resolution»
Axial resolution refers to the ability of a device or technique to distinguish and differentiate between objects or details that are located at different depths along the same line or axis. In simpler terms, it measures how well a system can identify and separate objects that are positioned one behind the other. Full definition
It is ideal for studying events in the plasma membrane of living cells with high axial resolution.
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Improvements of similar magnitude in imaging speed and a twofold gain in axial resolution relative to confocal microscopy yield 4D spatiotemporal resolution high enough to follow fast, nanoscale dynamic processes that would otherwise be obscured by poor resolution along one or more axes of spacetime.
On the other hand a confocal fluorescence microscope will provide an excellent axial resolution but exposes the specimen to high energy levels and might require several 10s of seconds for each image.
E.g. a conventional fluorescence microscope can easily produce ten bright images per second but does not provide axial resolution.
Multiple cell layers require a moderate axial resolution but still a high frame rate, which is provided by a spinning disc confocal fluorescence microscope.
In order to improve axial resolution and reduce phototoxicity for imaging live specimens, they combined light sheet microscopy with sequential multiple - view reconstruction (5).
The thinness of the sheet leads to high axial resolution and negligible photobleaching and background outside of the focal plane, while its simultaneous illumination of the entire field of view permits imaging at hundreds of planes per second even at extremely low peak excitation intensities.