Carlson B, Lahusen T, Singh S, et al: Down - regulation of cyclin D1 by transcriptional repression in MCF - 7
human breast carcinoma cells induced by flavopiridol.
Here we report that HB1.F3 NSCs can be engineered to express and secrete functional full - length HER2 - specific human immunoglobulin molecules that can selectively bind to and inhibit the proliferation of HER2 -
positive breast carcinoma cells.
To assess the ability of F3 - IgG to inhibit the proliferation of HER2 -
overexpressing breast carcinoma cells, we measured the proliferation of BT474, MCF7 / HER2 and MCF7 breast cancer cell lines in the presence of trastuzumab, F3 - IgG, or an isotype - matched control antibody.
Carlson BA, Dubay MM, Sausville EA, et al: Flavopiridol induces G1 arrest with inhibition of cyclin - dependent kinase (CDK) 2 and CDK4 in human
breast carcinoma cells.
Breast carcinoma cells (5 × 103 cells / well) were seeded into a 96 - well plate and treated with trastuzumab, isotype - matched control IgG, or F3 - IgG at concentrations ranging from 0.01 to 10 µg / mL.
Human embryonic kidney (HEK) 293T cells, and MCF7, and MDA - MB - 231
breast carcinoma cells were maintained in Dulbecco's modified Eagle's medium (DMEM) with 10 % FBS, 100 U / ml penicillin, 100 μg / ml streptomycin with or without 250 ng / ml amphotericin B (Invitrogen, Carlsbad, CA) and incubated at 5 % CO2 at 37 °C.