Live - cell analysis of virus - infected
cells by fluorescence microscopy or flow cytometry represents a promising approach to investigate virus - cell interactions.
Following preliminary proof - of - principle studies at Columbia University in models of inflammation, they were further tested in a clinically relevant disease model in mice and were shown to be capable of maneuvering through the blood circulation, and traversing leaky regions through to the inside of the plaques, as was
demonstrated by fluorescence microscopy imaging of the plaque lesions.
The bisbenzimide dye, Hoechst 33342 (5 μg / ml), was then added to stain the chromosomes and reveal the location of the nuclei, and Fluoromount - G was added to reduce fluorochrome quenching during analysis of the
slides by fluorescence microscopy.
Several methods to measure
FRET by fluorescence microscopy can be used, including fluorescence lifetime imaging, spectral imaging, donor fluorescence dequenching upon acceptor photobleaching.