In a job that is essentially 50 % drug development and 50 % discovery science, Goosney studies peptides that modulate innate immunity and
uses cell biology techniques and in vitro screens to determine which peptides are effective.
Besides learning
many cell biology techniques (tissue culture, Westerns, generating DNA constructs, transfections) as well as fly genetics, students can get familiar with imaging techniques depending on their own interests and experience.
Recent progress in phylogenomics, and the implementation of modern molecular, microscopy, and
cell biology techniques in a handful of spiralian model systems have made that possible.
The projects will provide training in: current molecular biology techniques (including RNA interference, genetic transformation, analyses of gene expression);
cell biology techniques (cell culturing, cell transfections, imaging); protein biochemistry (2D electrophoresis, protein functional analyses); and bioinformatics (structure predictions, phylogenetic analyses; molecular interactions).
This course is aimed at researchers who are familiar with basic molecular and
cell biology techniques and want to learn how to create an engineered mammalian cell line using the most recent and advanced CRISPR / Cas9 system.
We use structural approaches (i.e. X-ray crystallography, hydrogen - deuterium exchange, multi-angle light scattering), biochemical and biophysical methods (i.e. recombinant assays and reconstitutions, in vitro binding studies, fluorescence - based assays), and
cell biology techniques (i.e. immunofluorescence, immunoprecipitations) to study the mechanism of chromatin duplication, and we aim to understand how chromatin duplication affects cellular life and disease development.