We conclude that the Seqping pipeline predictions are more accurate than
gene predictions using the other three approaches with the default or available HMMs.
«Using improved computer software, we have
enhanced gene predictions and secondary metabolite gene cluster predictions in both species to a level not found in most other filamentous fungal species,» says Robert King, the team's lead bioinformatician at Rothamsted.
The goals of the project are as follows: (1) to shotgun sequence the C. murisgenome to 8X coverage; (2) to sequence 20,000 clones from a C. muris cDNA library to
provide gene prediction training and verification data; and (3) to manually annotate the C. muris genome sequence in comparison with the genome sequence data for C. parvum and C. hominis.
To address this, our team has released near complete genome assemblies and high quality, RNA - Seq -
guided gene predictions for two of the major C. auris clades via NCBI GenBank in advance of publication.
Chromosomes 2 and 4 have been completely sequenced and combed
by gene prediction computer programs.
Our structural annotation (
gene prediction) strategy involved masking repetitive DNA sequences, followed by a combination of EST and protein alignments, trained gene prediction algorithms, and homology - based gene predictions.
Our structural annotation (gene finding) strategy involved masking repetitive DNA sequences, followed by a combination of EST and protein alignments, trained
gene prediction algorithms, and evidence - based gene predictions.
Gene prediction is only meaningful in large populations (as evolutionary theory clearly implies).