Sentences with word «hemocytometer»

Beginning 72 hours post-stimulation, cells were counted every 48 hours using trypan blue dye exclusion on an automated hemocytometer (Countess, Invitrogen) and split to 0.8 × 106 with fresh media containing 100 U / ml IL - 2.

The pellet was resuspended (1 % bovine serum albumin in PBS), and total inflammatory cell numbers were determined via an improved Neubauer hemocytometer (ProSciTech, Thuringowa, Qld, Australia).

Following gesicle treatment, CD81 - negative hiPS cells sorted via flow cytometry were counted using a hemocytometer.

For the colony formation assay, cells were trypsinized to generate single - cell suspensions and counted by a hemocytometer.

At each 24 - hour interval, the cells were collected, stained with trypan blue, and counted with a hemocytometer.

Neurospheres were washed twice with 10 ml of medium, gently triturated, and counted on a hemocytometer.

The plated cells were then dissociated and counted using a hemocytometer in order to express results as picograms or nanograms of growth factor produced per day per million cells.

Cells were dissociated by trituration, counted on a hemocytometer and resuspended in conditioned medium at 1000 cells / µl.

At the end of this incubation, all rafts were thoroughly washed with PBS to remove any non-adherent cells, while adherent cells were trypsinized and counted using a hemocytometer.

* Lab Instruments: Oscilloscope, Function generator, digital multimeter, Basic circuit elements, spectrophotometer, microscope, flow cytometer, hemocytometer, ventilators.