Sentences with word «immunoaffinity»
Individual CSF samples from another set of 10 healthy volunteers, age 37 — 44 years (median = 40) and 5 women and 5 men, were analyzed by LC - MS analysis without immunoaffinity depletion.
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Due to the limit in sample volume, the CSF samples used in individual LC - MS analyses were not immunoaffinity depleted and fractionated, and therefore had much lower proteome coverage compared to the pooled samples.
The method uses a well - established technique called immunoaffinity capillary electrophoresis (IACE).
Instead of permanently depositing particles through immunoaffinity, anti-EpCAM coated magnetic microparticles were trapped over the untreated PDMS surface by an external magnetic field and were then released by readily removing the magnet for CTC collection.
The group uses immunoaffinity — the specific chemical affinity between an antibody and an antigen — to make CTCs adhere to the device while normal blood cells flow past.
Multi-component immunoaffinity subtraction chromatography: an innovative step towards a comprehensive survey of the human plasma proteome.
Combining immunoaffinity - based partitioning, SCX fractionation and LC - MS / MS, we identified approximately 30,000 peptides for each pooled sample corresponding to 2,783 nonredundant proteins in CFS patient samples and 2,768 proteins in nPTLS patient samples, compared to the 2,630 proteins present in the CSF of healthy normal control subjects.
Individual CSF samples from a group of 14 of the 25 nPTLS subjects (aged 25 — 58 years with a median age of 48 years, 6 female and 8 male) were also used in direct LC - MS analysis without immunoaffinity depletion.
Individual CSF samples from 14 of the 43 CFS subjects (aged 33 — 48 years with a median age of 43 years, 7 female and 7 male) were also used in direct LC - MS analysis (i.e., no MS / MS was performed) without immunoaffinity depletion.
The bound fraction of abundant proteins from the immunoaffinity depleted flow through fraction was analyzed separately and included in the subsequent analysis.