Sentences with word «immunostaining»

(C and D) Average lesion number (C) and area (D) were quantified using images of immunostaining for RCAS - caErbb2 - HA.

(B - G) Anti-CCHa2 immunostaining of the fat body (B - E) and gut (F, G) from wild - type and CCHa2 mutants.

The morphological identification of eosinophils in H&E stains was supported by using Giemsa stain to highlight intracytoplasmic granules in selected lung sections (not shown), and confirmed by immunostaining with antibodies against mouse eosinophil major basic protein (provided by the Lee Laboratory, Mayo Clinic, Arizona)[36].

We assessed microgliosis by immunostaining for CD68 and found significantly elevated CD68 immunoreactivity in the frontal cortex, hippocampus, and thalamus of Grn − / − mice, as expected (Fig. 6A w - y).

Target protein analysis using immunostaining and confocal microscopy.

Image shows strong immunopositivity at the membranes of cardiomyocytes (invaginations of immunostaining show points of branching of each myocyte).

Analysis of CD68 immunostaining in the frontal cortex revealed an effect of genotype (ANOVA, p < 0.0001) and a strong trend for an exercise effect (ANOVA exercise effect, p = 0.051).

Global analysis of CD68 immunostaining revealed a region x genotype interaction (p < 0.0001), so each region was analyzed with a separate ANOVA.

When the brains of the mice were examined via immunostaining after treatment, the animals had, on average, a few dozen cancer cells left, compared with tens of thousands of cancer cells in animals that received a control treatment.

They then successfully generated custom antibodies to each of these proteins and confirmed that there was no cross-reactivity, opening the door for immunostaining analysis.

Embryos were cultured to the indicated developmental stage and 12 - µm, cryostat sections were immunostained as previously described [39].

Immunostaining reveals Borreliae within XT from both treated and untreated monkeys.

The inset shows positive immunostaining with CD45R / B220 (brown stain); nuclei were counterstained with hematoxylin.

The STOP cassette includes a mutant YFP that does not fluoresce, but can be detected via immunostaining.

Quantification of HNF4α immunostainings showed that over 90 % of hiPS cell - derived hepatocytes differentiated using our protocol express HNF4α [4].

Significant reduction in H3K9 dimethylation at 100nM in MDA - MB231 cells as measured by fluorescence immunostaining without significant cellular toxicity.

During sperm biogenesis, flagella appear tightly bundled, which makes it more challenging to get accurate measurements of protein levels from immunostained specimens.

Over the summer I have been optimizing tissue - clearing techniques for 3D immunostaining and optical imaging to understand the foreign body response to implanted devices in the brain and adrenal gland.

Immunostaining intensity for collagen was quantified by determining the reciprocal intensity using Image J software [23].

To determine whether SIV infection leads to corneal nerve fiber loss, the researchers immunostained corneas for the nerve fiber marker βIII tubulin.

«Immunostaining conducted with skin samples collected from dogs with the disorder proved that the functional form of the protein, or collagen, was absent from the skin of these dogs,» says Kati Dillard, a veterinarian at the Finnish Food Safety Authority Evira.

The brain neuropil is marked by anti-bruchpilot immunostaining (magenta).

Increased tight junction immunostaining 1 day after blast injury due to dexamethasone (DEX) treatment.

«We suspected there was a small number of smooth muscle cells we were failing to identify using the typical immunostaining detection methods.

Professor Zhang's team were able to visualize the pinning, stretching and rupturing of cervical cancer cells by immunostaining different parts of the cells and viewing them under a confocal fluorescent microscope and a high - resolution scanning electron microscope (SEM).

To achieve this goal, her lab has developed technology platforms for large - scale immunostaining and systematic validation of antibody specificity, and has initiated citizen - science efforts to refine the Human Protein Atlas database.

Tumor spots were excluded from the analysis if they had abundant necrotic tissue, insufficient tumor cells, loss of tissue in the spot during immunostaining, or showed high background on the negative control slide (incubated with secondary antibody but not with anti-B7H1 antibody) due to high endogenous biotin.

A multiple immunostaining technique is a combination of individual antigen detections in order to study the expression and co-localization of two or more different antigens in the same sample.

Free - floating immunostaining was done following well - established protocols [10, 11, 34, 35].

To characterize the NPCs, immunostaining experiments were conducted with antibodies against NPC markers, including Sox2, Nestin and Olig2.

When the transgenic larval brains were co-stained with anti-Dilp2 antibody, CCHa2 - R > nlsGFP colocalized with the Dilp2 immunostaining (Fig 2H).

H3K9Me2 immunostaining and cell viability measured in MDA - MB231 cells after 48h exposure to BIX - 01294 or UNC0638.

(A) Retinal section obtained from a P150 eye immunostained with human - specific nuclear marker.

Wholemount immunostaining was carried out on mouse tail epidermis.

Representative images (20 ×) are shown of doublecortin immunostaining in the dentate gyrus.

(D) Sections of EBs grown under the same conditions as in part (C), showing that the outer layer consists of visceral endoderm as marked by Gata6, FoxA2, and Sox17 immunostaining (shown as red).

Cell types present in each well were then categorized and quantified based on phenotype and GCTM2 immunostaining (indicated by AP positive red color).

Methods: We performed immunostaining to assess the localization of PRCP in arcuate Neuropeptide Y / Agouti - related Peptide (NPY / AgRP) neurons.

A surprising finding of our newly constructed UAS - DTH1m is that it expresses DTH at an undetectable level when regulated by dopaminergic GAL4 drivers even when fully rescuing DA, indicating that DTH immunostaining is not necessarily a valid marker for DA expression.

Using immunostaining, the researchers counted the remaining tumor cells; the mice treated with GD2 CAR - T cells had a few dozen remaining cancer cells per animal, while each control mouse had tens of thousands of cancer cells.

For clonospheres, single cell suspension post treatment were cytospun (Zettrek) on poly - l - lysine coated slides at 1100 rpm for 7 min at RT, fixed for 15 minutes with 4 % paraformaldehyde and immunostained as mentioned above.

HNF4α immunostaining showed over 90 % of hepatocytes differentiated with our protocol expressed HNF4α (Asplund et al. 2016).