Sentences with word «lentivirus»

B, CSCs isolated from 231BrM infected with lentivirus of miR7 or KLF4 expression plasmids were seeded on top of mouse brain endothelial cells or Matrigel.

This is a model of a retrovirus capsid hexamer, showing the conserved beta - hairpin domains common to most kinds of retroviruses (circled) and a pocket containing additional sites thought to affect recognition of lentiviruses by the TRIM5 anti-viral defense proteins of old world monkeys (arrow).

Let us know how you use lentivirus in the comments section below!

A, 231BrM was infected with KLF4 or control lentivirus and the CSC population (CD24 - CD44 + ESA +) was measured by FACS after 72 hours.

Because of its unique specificity for retroviruses (whereas other restriction factors often have broader antiviral activity), the researchers hypothesized that the evolution of TRIM5 in African monkeys should reveal selection by lentiviruses closely related to modern SIVs.

A third - generation lentivirus vector with a conditional packaging system.

The most well - known lentivirus in humans is HIV.

We found that the percentage of the EdU - positive cells in the GFP - positive organoids was significantly reduced when the human BCSCs were infected with the anti-miR-142-3p-expressing lentivirus (Figure 4C).

CSCs were prepared from 231BrM and CN34BrM cells that were infected with lentivirus carrying with or without miR -7-2, and they were transplanted into nude mice through intracardiac injection followed by monitoring metastatic tumor growth in the brain.

B, 231BoM - 1833, 231BrM - 2a, CN34 - BoM2d, CN34 - BrM2c, and MCF7 - BoM2d were infected with lentivirus expressing pri - miR -7-1, pri - miR -7-2, or vector only and the KLF4 mRNA levels were measured by RT - PCR.

They identified a cluster of adaptive changes unique to the TRIM5 proteins of a subset of African monkeys — the Cercopithecinae, which include macaques, mangabeys, and baboons — that suggests that ancestral lentiviruses closely related to modern SIVs began colonizing primates in Africa as far back as 11 - 16 million years ago.

This family of viruses, known as Lentiviruses (or «slow virus») is species - specific.

Human embryonic kidney (HEK) 293T cells, a packaging cell line for lentivirus production, and the preosteoclast cell line RAW264.7 (ATCC) were maintained in DMEM supplemented with 10 % FBS and antibiotics.

The antisense genes were inserted into the cells» nuclei by a modified lentivirus that had been crippled to ensure it was incapable of reproducing.

To induce cancer mutations, the investigators package the genes for Cas9 and the RNA guide strand into viruses called lentiviruses, which are then injected into the target organs of adult mice.

To begin, the team used harmless lentiviruses to distribute the genes for about 100 million distinct antibodies among a similar number of HeLa cells, and later exposed the cells to the rhinovirus.

Development of a self - inactivating lentivirus vector.

For example, while freeze thaw is generally not recommended, Holic et al. found that lentivirus produced at pH 6 are resistant to multiple freeze thaw cycles.

When using the ClonTracer system, lentiviruses containing these barcodes infect the target cell population at low multiplicity of infection (MOI) such that each individual cell receives only one barcode.

We further confirmed that the protein level of APC was elevated in the human breast cancer xenograft formed by the human BCSCs transfected with the anti-miR-142-expressing lentivirus (Figure 7B), which is consistent with our observations using the breast cancer cell lines.

We further confirmed that the protein level of APC was elevated in the human breast cancer xenograft cells transfected with the anti-miR-142-3p lentivirus (Figure 7B).

Mammary cells infected with the miR -150-expressing lentivirus formed a hyperplastic mammary tree with extremely increased branching and thick mammary ducts (Figure 6A, C).

The researchers administered these mice with lentiviruses targeting three different genes, allowing them to see how each gene cooperates with Kras to influence tumor growth.

Its product, the TRIM5 protein, interacts directly with the outer shell of lentivirus particles after they enter the host cells and prevents the virus from multiplying there.

To examine the effect of miR - 7 on the expression of KLF4, we ectopically expressed miR -7-1 or miR -7-2 in 231BoM and 231BrM cells by infecting corresponding lentivirus expression vector followed by qRT - PCR analysis.

Researchers typically use viruses for two types of studies --(1) knock - in; introducing a protein - coding gene into cells to study its function, or (2) knockout / knockdown; studying gene function through deletion (potentially using CRISPR lentivirus knockout libraries) or reduction of gene expression, respectively.

Lentiviruses allow widespread and conditional manipulation of gene expression in the developing mouse brain.

Lentiviruses pseudo-typed with the VSV - G envelope protein use the LDL receptor to infect cells and have a broad host range.

Many labs try to use freshly collected lentivirus for downstream applications.

When harvesting lentivirus, some labs prefer to collect and pool the harvests from multiple time points while others opt for a single harvest after transfecting with the transfer and packaging plasmids.

It has most commonly been used on retrovirus and lentivirus factors.

After initial experiments confirmed that the approach was working, Marumoto injected lentiviruses carrying two well - known oncogenes, H - Ras and Akt, into three separate brain regions of mice lacking one copy of the gene encoding the tumor suppressor p53: the hippocampus, which is involved in learning and memory; the subventricular zone, which lines the brain's fluid - filled cavity; and the cortex, which governs abstract reasoning and symbolic thought in humans.

We crunched the numbers on our Viral Service (and then we crunched them again) to find the most requested lentivirus and AAV of 2016.

We use a range of approaches to study these cell processes, including a mammary gland regeneration model in which mammary stem cells are transduced with lentivirus then transplanted into the cleared fat pads of recipient mice, where the cells regenerate an entire new mammary ductal tree.

Stable trastuzumab - expressing NSCs (HB1.F3.Lenti - H2IgG) were generated by incubating HB1.F3 cells with purified lentivirus (MOI 100) for 4 h in the presence of 8 µg / mL polybrene.

Macron email leak dating site - LIVE VIDEO chat - BEST and and acquired Dating Ontario lentivirus a subgroup to the aftermath Zoe Brennan on the capture of.

We also perform infectious disease testing (tuberculosis, brucellosis, Q - fever, caseous lymphadenitis, small ruminant lentivirus) for herd biosecurity and milk certification.

B, CSCs were isolated from 231BrM infected with KLF4 or control lentivirus and cultured in low - attachment plates.

To confirm that these findings are applicable to the human BCSCs, we infected the human breast cancer cells with the anti-miR-142-3p-expressing lentiviruses and evaluated the ability to form the organoids derived from the human BCSCs.

Advantages of lentivirus include a large genetic capacity and the ability to transduce both dividing and non-dividing cells.

To deliver the genes for Cas9 and the RNA guide strand, the MIT team packaged them into viruses called lentiviruses, which can be injected into the target organs of adult mice.