Sentences with word «ligase»

The role of Smurf1 — one of hundreds of E3 ubiquitin ligases in mammals — was unknown in this process.

Hsia HE, Takeda M, Courchet J, Goebbels S, An W, Eickholt B, Polleux F, Rotin D, Wu H, Nave KA, Rossner M, Brose N, Kawabe H. (2014) The Ubiquitin E3 Ligase Nedd4 - 1 acts as a downstream target of PI3K / PTEN - mTORC 1 signaling to promote neurite outgrowth.

We are especially interested in the role of Cullin - RING complexes, the largest class of E3 ubiquitin ligases in eukaryotes.

Previously, the Doherty group at the University of Sussex's Genome Damage and Stability Centre discovered that mycobacteria repair DNA breaks using an enzyme complex called Ligase D (LigD).

The MDC researchers succeeded in increasing the efficiency of the more precisely working HDR repair system by temporarily inhibiting the most dominant repair protein of NHEJ, the enzyme DNA Ligase IV.

Eric H. Eckland, Jack W. Szostak and David P. Bartel, «Structurally Complex and Highly Active RNA Ligases Derived from Random RNA Sequences» [abstract], doi: 10.1126 / science.7618102, p 364 - 370 v 269, Science, 21 July 1995.

To understand how Parkin and other RBR ubiquitin ligase enzymes achieve this, EMBO Young Investigator David Komander and his coworker Tobias Wauer crystallized a form of human Parkin and used X-ray diffraction patterns to determine how the Parkin protein chain folds into a three - dimensional structure.

The researchers have observed, for the first time, how D - cycloserine inhibits the D - alanine - D - alanine ligase enzyme.

Proteins were expressed as soluble monobiotinylated proteins by transient cotransfection of HEK293E cells with the BirA biotin ligase [34] and harvested six days post transfection [35].

RPM - 1 uses PPM - 2, an enzyme that removes a phosphate group from a protein thereby altering its function, in combination with ubiquitin ligase activity to directly inhibit DLK - 1.

24) Thu KL, Pikor LA, Chari R, Wilson IM, MacAulay CE, Tsao M, Gazdar AF, Lam S, Lam WL, Lockwood WW (2011) Disruption of KEAP1 / CUL3 E3 ubiquitin ligase complex components is a key mechanism of NF - kappaB pathway activation in lung cancer.

Following electroporation into R26 BirA cells, a cell line that expresses bacterial BirA ligase from the ROSA26 locus, hygromycin resistant clones (200 µg / ml) were expanded for Southern analysis to identify correct targeting events.

Haploinsufficiency of the E3 ubiquitin - protein ligase gene TRIP12 causes intellectual disability with or without autism spectrum disorders, speech delay, and dysmorphic features.

The IGF - 1 / PI3K / Akt pathway prevents expression of muscle atrophy - induced ubiquitin ligases by inhibiting FOXO transcription factors

In ligase deficient mice, growth of mouse melanoma cells in vivo is attenuated, while tumor infiltration by CD4 + / CD8 + T cells and dendritic cells is increased.

«But we also used a trick of nature and blocked Ligase IV with the proteins of adeno viruses.

Tyrosine ligase adds a C - terminal tyrosin to monomeric alpha tubulin.

The E3 ligase VHL promotes group 2 innate lymphoid cell maturation and function via glycolysis inhibition and induction of interleukin 33 receptor.

The Phr1 ubiquitin ligase promotes injury - induced axon self - destruction.

In normoxic cells, the transcription factor HIF - 1α is degraded by the action of E3 ubiquitin ligase on the oxygen - dependent degradation domain (ODD) of HIF - 1α; however HIF - 1α is stabilized and therefore, accumulates in hypoxic cancer cells.

@Poltergeist — Not precisely sure of the intent of the original evolved ligase post or your response, but is it safe to say that this study, as with other in vitro evolution research, is proof of concept, i.e. random variation and selection can and does yield novel functionality?

EDD1 is an E3 ubiquitin ligase involved in degrading cellular proteins, while TIP60 act as a tumor suppressor protein, and can both be found in the human body.

The increase in JAK1 was due to low levels of the protein that regulates its expression, ubiquitin ligase RNF125.

The team then used high throughput imaging to see what ligases increase and decrease the levels of their favorite clock proteins in cells.

The researchers hope that by applying this new method, more ligase drug targets can be found and developed into new therapies across the spectrum of health challenges.

Certain ligases, like Fbxl3, can be targeted with small molecules.

«This exquisite regulation of an ubiquitin ligase has not been previously described and is very exciting for the field of ubiqutin biology.»

The latest study establishes that a closely related protein apparatus called Ligase C, whose function was unclear until now, combines with other repair proteins to fix damaged DNA bases in mycobacterial genomes caused by the attack of oxygen free radicals.

«We could also show that these inhibitors affect related RBR ubiquitin ligases such as HOIP, which is important for inflammatory immune responses.»

Ubiquitin ligase TRIM3 controls hippocampal plasticity and learning by regulating synaptic?

Zheng is now trying to translate this finding to human pathology, where mutations in human ubiquitin ligases cause low affinity bindings to target proteins.

This effect is mediated by an enhanced interaction of H2AX with the E3 ubiquitin ligase RNF168, which is associated with H2AX poly - ubiquitination and promotes its degradation by the proteasome.

SUMO ligases conjugate the protein, via its C - terminal carboxylate, to the side - chain lysine of target proteins to generate an isopeptide linkage.

Structure and function of the first full - length murein Peptide ligase (mpl) cell wall recycling protein.