Sentences with phrase «luciferase assays»

Protein was extracted into 200 μl of Luciferase Cell Culture Lysis Reagent (Promega), and 40 μl of protein extract was used for luciferase assays.

The cells were cotransfected with the reporter gene constructs and related expression plasmids as indicated by using SuperFect (Qiagen) followed by transcriptional activity assay using Dual - Luciferase Assay System (Promega) according to the manufacturer's instruction.

His team looked at an experimental drug called PTC124, which the firefly luciferase assay had identified as a promising candidate for treating stop - sign forms of cystic fibrosis and which is now being tested in clinical trials.

D, IRF - 9 — deficient U2A cells were transiently transfected with IRF - 1 or empty vector, the binding of exogenous IRF - 1 to RIG - G gene promoter was tested by ChIP assay, and the induction of reporter gene pXP2 (− 310) was detected with luciferase assay.

Transfected cells were harvested at 48 hours posttransfection and a dual luciferase assay was done according to the instruction of the manufacturer.

Transfection and luciferase assay.

Luciferase assay was done at 48 hours posttransfection.

The luciferase assay was done at 48 hours posttransfection.

Larvae were staged at the onset of the L3, and fed on normal food for 24 h. For the luciferase assay, we used the entire bodies of five larvae.

Luciferase assays were performed using the Luciferase Assay System (Promega) according to the manufacturer's instructions.

FRE - luciferase assays were performed as described previously [35] with a minor modification.

Solberg N, Krauss S (2013) Luciferase assay to study the activity of a cloned promoter DNA fragment Methods Mol Biol, 977, 65 - 78 PubMed 23436354

Luciferase assays were analyzed based on the ratio of Firefly / Renilla to normalize cell number and transfection efficiency.

In addition to the fact that APC is among the top list of the comprehensive Ago IP / microarray experiments, our results of the luciferase assays, Western blots including the analyses using the anti-miR-142-3p, and TOP Flash experiments, indicate that miR - 142 strongly upregulate the activity of the canonical Wnt signaling pathways.

Davis Heart and Lung Research Institute, Columbus • OH 2008 — 2010 Part - Time Research Assistant Instrumental in total RNA isolation from cultured human and animal cells, real - time PCR, plasmid purification, western blotting and dual luciferase assay.