Sentences with phrase «lung fibroblasts»
"Lung fibroblasts" refers to cells in the lungs that play an important role in the structure and healing of the lung tissues. They produce proteins and connective tissue that help support the lungs and can repair damage when necessary. Full definition
(A) Human lung fibroblast cells were plated in 384 - well Optilux plates and screened with compound libraries at 15 µM for 18 hours.
journals.plos.org
Forkhead Box F1 represses cell growth and inhibits COL1 and ARPC2 expression in lung fibroblasts in vitro.
iPS cells, derived from the IMR - 90 human foetal lung fibroblast cell line [18], were cultured in stem cell medium lacking bFGF to encourage the spontaneous differentiation of cells (Fig. 1A).
This induced pluripotent stem cell (iPSC) line was generated from IMR90 fetal lung fibroblasts by viral transduction of a combination of OCT4, SOX2, NANOG, and LIN28 genes.
A recent report demonstrated a significant increase in COX2 expression in normal lung fibroblasts when exposed to cigarette smoke extracts [46].
Normal human lung fibroblasts (NHLF) were purchased from Lonza and maintained in FBM medium supplemented with 2 % FCS plus the supplied FGM - 2 SingleQuot components (insulin, hFGF - B, and antibiotic / antifungal agents).
Normal human lung fibroblasts (NHLF) cells grown in 384 - well Optilux plates were treated with candidate compounds and incubated for 18 hours prior to fixation and staining with Hoescht dye and anti-HMOX1 antibody as described in Materials and Methods.
In this report, normal human lung fibroblasts were used to screen a collection of synthetic small molecules for their ability to induce HMOX1.
Based on these observations, we report the development of a cell - based screening strategy to identify compounds that specifically modulate the expression of HMOX1 in normal human lung fibroblasts.