Sentences with word «lysis»

Reactions were terminated with 1 ml of ice - cold PBS, and cells were centrifuged and resuspended in 50 μl of cell lysis buffer containing 1 % NP - 40 and protease inhibitors.

The classic radiographic signs of discospondylitis are collapsed intervertebral disc space with lysis of the adjacent vertebral end plates and variable endplate sclerosis.

Cell pellets were resuspended in 1 ml cell lysis buffer [5 mM Pipes pH 8.0, 85 mM KCl, 0.5 % NP - 40] containing protease inhibitors and incubated for 10 min on ice.

After an overnight incubation at 4 ° C, for thorough stabilization, samples were homogenized in lysis buffer and total RNA was isolated using the GE Illustra RNAspin Isolation Kit (GE Healthcare) according to the manufacturer's protocol.

Instead, it relies on endogenous tumor antigens that are released during tumor lysis by treatments such as chemotherapy or local radiation.

Instead of targeting specific tumor antigens, this approach relies on endogenous antigens (including neoantigens) released during tumor lysis by treatments such as chemotherapy or local radiation.

Lyse cells with as small a volume volume of RIPA lysis buffer as possible before diluting the lysates with 1x PBS to the desired final volume.

Antibiotics should be continued until bone lysis resolves completely.5

All of our patients had some sort of tumor lysis syndrome.

«Our original plan was to understand the fate of organic carbon released from viral lysis of picocyanobacteria» said Feng Chen.

Using a multichannel pipette, transfer 25 µL / well of direct lysis buffer (from Step 2) to the required number of 96 well PCR plates.

Histopathology or cytology of the area to determine the reason for lysis.

A2, a capsid protein of RNA phage Qβ, is also responsible for host lysis.

Take, for example, the definition of bacteriophage: «any virus that infects and replicates within a bacterium, producing transmissible lysis or becoming lysogenic; usually species - specific».

Analytical flow cytometry analysis was performed on whole blood after lysis of erythrocytes and fixing in 2 % PFA.

After extensive washing with immunoprecipitation lysis buffer, the immunoprecipitated proteins were analyzed by immunoblotting using specific antibodies against DDX3 and FLAG epitope (ab1257, Abcam, Cambridge, United Kingdom).

Protein was extracted into 200 μl of Luciferase Cell Culture Lysis Reagent (Promega), and 40 μl of protein extract was used for luciferase assays.

The cells were incubated for 15 — 30 minutes at room temperature to allow complete cell lysis before determining luminescent levels using in an Envision plate reader.

Nuclei were pelleted by centrifugation (5000 rpm for 5 min) and resuspended in 350 µl nuclear lysis buffer [50 mM Tris pH 8.1, 10 mM EDTA, 1 % SDS] containing protease inhibitors.

Our estimates are also lower than rates of CTL lysis calculated (albeit using very different methods) for other virus infections which range from 1 d − 1 for HTLV - I to 12 d − 1 for acute LCMV [39 — 42].

It is worth noting that diabetic women had a higher prothrombotic fibrin profile, with denser fibrin clots and prolonged fibrinolysis, which was shown in increased maximum absorbance, clot formation, and lysis time.

Beta - glucanase promotes complete digestion of cereals and grains as well as lysis of yeast cell walls.

Cells were then lysed by adding 50 µL Lysis Buffer (provided).

Following lysis and solid - phase capturing of mRNAs with streptavidin magnetic beads, samples were reverse transcribed using random primers.

Once the cells were trapped, researchers observed how they underwent lysis: their cell walls and membrane ruptured, the cytoplasm leaked, and the cell disintegrated.

Most of those errors stem from the cell - lysis protocols scientists have been using.

«So in some cases, such as with our work on lysis in bacteriophages, precision in timing is obtained with no feedback at all.»

Recently, we showed that bortezomib treatment in mice bearing breast and kidney tumors provided host survival benefit by amplifying tumor cell caspase8 activation, CD8T cell effector molecules via increased NotchNFkB crosstalk and Fas ligandmediated lysis of tumor cells.

The nuclear pellet was resuspended in a second lysis buffer containing 50 mM HEPES (pH 7.9), 0.4 M sodium chloride, and 1 mM EDTA, with protease inhibitors (20 µg / ml aprotinin, 5 µg / ml leupeptin, and 1 mM PMSF).

Cytotoxicity was determined by the amount of 51Cr released by the target cells and the specific lysis was calculated as follows:

Tumors can suppress CD4 + T cell activity and CTL tumor lysis directly through secretion of immunosuppressive factors including TGF - β1 but also PGE - 2, and IL - 10.

However, because M. barkeri has a complex cell wall, produces a thick outer layer of polysaccharide, and grows in large aggregates of cells rather than as individual cells, standard RNA extraction protocols give incomplete lysis of cells and result in low yields of poor - quality RNA.

Different stages of pigment lysis were detected under the electron microscope.

Splenocytes were obtained following single - cell suspension and RBC lysis.

NHLF cells transfected with siRNA molecules were lysed in High Salt ELB lysis buffer [1 M Tris pH 8.0, 1 % NP - 40, 250 mM NaCl, 5 mM EDTA] supplemented with protease and phosphatase inhibitors (1x G - Biosciences Protease Arrest, 200 µM Na3VO4, and 1 mM PMSF).

Cells were lysed with TN1 lysis buffer (50 mM Tris, 125 mM NaCl, 1 % Triton X-100, 10 mM EDTA, 10 mM sodium fluoride and 10 mM sodium pyrophosphate) supplemented with protease inhibitor cocktail (1.2 mM AEBSF, 0.46 μM aprotinin, 14 μM bestatin, 12.3 μM E-64, 112 μM leupeptin, 1.16 μM pepstatin)(Amresco; Solon, OH)-RRB- and 1 mM sodium orthovanadate (Enzo Life Sciences; Farmingdale, NY).

For this protocol, samples were lysed in 1 × RIPK2 lysis buffer [50 mM Tris (pH 7.5), 0 mM MgCl2, 1 % Triton X-100, 1 mM dithiothreitol, 1 mM EDTA, 1 mM EGTA, with freshly added 1 mM β - glycerophosphate and protease inhibitors (phenylmethylsulfonyl fluoride and aprotinin)-RSB- and immunoprecipitated overnight using 1.5 μg rabbit anti-RIPK2 antibody.

The new protocol incorporates optimised lysis and hypotonic buffers and guidelines on how to achieve maximum extraction of chromatin from limited sample quantities.