Sentences with word «oligonucleotide»
An oligonucleotide is a short chain of molecules called nucleotides. Nucleotides are the building blocks of DNA and RNA, which are molecules that carry genetic information. So, an oligonucleotide is a small sequence of these building blocks that scientists and researchers use to study and manipulate genes and genetic material. Full definition
The structure of this enzyme was elucidated by isolating clones from a bovine brain complementary DNA library through the use of oligonucleotide probes derived from partial amino acid sequence.
science.sciencemag.org
Washington University holds joint patent applications with Ionis Pharmaceuticals on the use of oligonucleotides for reducing tau levels.
Since complex oligonucleotide treatment comes with many challenges, researchers have begun to explore genome editing approaches for exon skipping.
But treating patient - derived B cells with CpG oligonucleotides stimulates every B cell in the sample, not just the tiny fraction capable of producing a particular antibody.
«Optimization of RNA isolation from the archaebacterium Methanosarcina barkeri and validation for oligonucleotide microarray analysis.»
«Thirty years of innovation pays off as oligonucleotide therapeutics come to market: Approval of effective products follows predictable patterns of innovation.»
If you are looking to insert small DNA sequences (< 200bp), using a DNA oligonucleotide as donor DNA should do the trick.
The present work shows that the approval of oligonucleotide therapeutics exhibits a similar pattern.
Alternatively, the gRNA, Cas9, and donor oligonucleotide can also be electroporated into the mouse zygote -LRB-
Biological and technical replicates for each population were hybridised to NIA Mouse 44K Microarray v2.3 (whole genome 60 mer oligonucleotide probe; manufactured by Agilent Technologies, # 014951)[36].
The mRNA competitively hybridizes with the DNA oligonucleotides on the nanocomplex, and it is more attracted to this DNA than are the existing reporter genes.
Mongersen is Celgene's an anti-inflammatory, anti-sense oligonucleotide, which is being studied to treat Crohn's disease, which is a chronic inflammatory bowel...
WVE - 120101 and WVE - 120102 are investigational stereopure antisense oligonucleotides designed to selectively target the mHTT mRNA transcript of SNP rs362307 (SNP1) and SNP rs362331 (SNP2), respectively.
Dilutions of the purified and pooled PCR amplicons were made and quantified using a synthetic oligonucleotide standard of known molarity [35], to approximate an optimal bead to template ratio for emulsion PCR.
Graham et al. showed that lowering ApoC - III using antisense oligonucleotides reduces plasma TGs in rodents, nonhuman primates, and humans (15).
Lorson's study, «Morpholino antisense oligonucleotides targeting intronic repressor Element1 improve phenotype in SMA mouse models,» was published in September 2014 in the Journal of Human Molecular Genetics.
It's trickier for oligonucleotide approaches, such as Isis's antisense therapy, which, in its first phase 1 study, was administered only into the CNS.
Walton et al. «Prediction of Antisense Oligonucleotide Binding Affinity to A Structured RNA Target», Biotechnology and Bioengineering, 65 (1): 1 - 9, 1999.
A small catheter is then implanted into the area surrounding the spinal cord, in order to pump antisense oligonucleotide drugs directly into the nervous system.
«He went out and he found somebody who was, at a very early stage, working on novel oligonucleotide synthesis technology, and bought it.
This panel combines new generations of FISH technologies, pairing oligonucleotide - based SureFISH technology with a ready - to - use formulation of formamide - free IQ Hybridization Buffer, resulting in high signal - to - noise ratios with less than 4 hours of turnaround time.
Overlapping «cassettes» of 5 to 7 kilobases (kb), assembled from chemically synthesized oligonucleotides, were joined by in vitro recombination to produce intermediate assemblies of approximately 24 kb, 72 kb («1/8 genome»), and 144 kb («1/4 genome»), which were all cloned as bacterial artificial chromosomes in Escherichia coli.
Shortening these RNAs enables Integrated DNA Technologies to manufacture the components as high - quality, synthetic RNA oligonucleotides that elicit less toxicity and innate immune response activation in cells.
The sunY ribozyme and its small selected variant can both catalyze template - directed oligonucleotide assembly.
In the second technique, the presence of the beta A and beta S alleles is determined by restriction endonuclease digestion of an end - labeled oligonucleotide probe hybridized in solution to the amplified beta - globin sequences.
We examine here using high - density oligonucleotide array the state of DNA methylation, histone acetylation and gene expression in a 7 million base pair region of chromosome 18 containing the NR3C1 gene in the hippocampus of adult rats.
After that, the droplets merge with another set of droplets containing oligonucleotide barcodes.
Several different oligonucleotides can thus be produced at the same time in a perfectly balanced combination, which is important if they are to be crystallised or used therapeutically.
Such oligonucleotides can be designed to target the RNA for almost any protein.
The biotech company, however, will pursue next generation antisense oligonucleotides research.
«This core is functionalized with a dense layer of stable dithiol - modified DNA oligonucleotides hybridized with distinctive fluorophore - capped «reporter» sequences.»
The double strand breaks are created either by site - specific designer zinc finger endonucleases or triple helix forming oligonucleotides that are able to recognise and induce repair of specific mutations in the genome.
Develop KI techniques using modified oligonucleotides in cultured cells and in model organisms
Clinical trials have used oligonucleotide exon skipping (OEN) to remove mutated exons from the dystrophin transcript.
Stoke Therapeutics Expands Leadership Team, Building on Deep Expertise in Oligonucleotide Chemistry and Rare Disease Research and Development
The droplets then mix with gel beads, each of which carries a unique oligonucleotide «barcode.»
The effect of the injection of the antisense oligonucleotides lasted about 16 weeks.
Luckily, Turner took a sabbatical at the University of Colorado, and down the hall was another young scientist, a Polish researcher by the name of Ryszard Kierzek, who was using organic chemistry to produce four oligonucleotides at a time, and as many as two sets of four in a day.
A DNA array detection method is reported in which the binding of oligonucleotides functionalized with gold nanoparticles leads to conductivity changes associated with target - probe binding events.
Full - length poliovirus complementary DNA (cDNA) was synthesized by assembling oligonucleotides of plus and minus strand polarity.
The researchers administered a dose of the anti-tau oligonucleotide to 9 - month - old mice every day for a month and then measured the amount of tau RNA, total tau protein and tangles of tau protein in their brains when the mice were 12 months old.
Antisense oligonucleotides restored the production of normal protein in all cases.
TNAs -LSB-(l)- α - threofuranosyl oligonucleotides] containing vicinally connected (3 ′ → 2 ′) phosphodiester bridges undergo informational base pairing in antiparallel strand orientation and are capable of cross-pairing with RNA and DNA.