Sentences with word «polyclonal»
The word "polyclonal" refers to a group of different antibodies that target various specific parts of a foreign substance, such as a virus or bacteria. It can be thought of as a diverse team of antibodies working together to fight off invaders. Full definition
This option is useful when the primary antibodies are from different hosts (e.g., a mouse monoclonal antibody against antigen A and a rabbit polyclonal antibody against antigen B).
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LGALS3 MaxPab rabbit polyclonal antibody.
Grifols is a world leader in production of polyclonal antibody drugs.
A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody.
Initially, we stained the XT midgut smear using DFA with polyclonal anti-Borrelia species antibodies.
ab150077, an Alexa Fluor ® 488 - conjugated goat anti-rabbit IgG polyclonal (1/1000), was used as the secondary antibody.
Secondary antibody 2: Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor ® 594) pre-adsorbed (ab150084), 1:300 Nuclei were counterstained with DAPI
Since validated XMRV - positive human sera are not currently available, we used experimentally derived polyclonal antisera and monoclonal antibodies to assess antigenic reactivity of the WB assay.
Control mice were treated with PBS only, a control mAb or polyclonal human IgG.
GigaGen is advancing a pipeline of first - in - class biotherapeutics, including novel oncology antibody drug combinations and the world's first recombinant polyclonal hyperimmune gammaglobulin.
The development of anti-galectin 3 polyclonal responses in pancreatic cancer patients after vaccination with GVAX (2) or prostate cancer patients following Sipuleucel - T (3) has been associated to prolonged survival, thus providing an supportive rationale for targeting galectin 3.
In addition to staining the midgut contents with FITC - labeled polyclonal anti-Borrelia species antibody, we washed and re-stained this set and stained the second set of xenodiagnostic tick (XT) samples with an anti-OspA monoclonal antibody (CB10, obtained from J. Benach [41]-RRB-, followed by anti-mouse IgG - Alexa 488 (Molecular Probes).
Ehrlichia infection in dogs and FIP disease in cats, canine heartworm infection, leishmania infection, chronic liver disease, lupus and other auto - immune type diseases, response to chronic infections and inflammations of many kinds, chronic and severe dental (periodontal) disease, Cancer of the cells that produce one or several specific antibodies (monoclonal or polyclonal gammopathies) myeloma can all cause high blood globulin levels.
A highly specific polyclonal rabbit antiserum directed against murine cachectin / tumor necrosis factor (TNF) was prepared.
These pathogenic effects of physiologically relevant amounts of NS1 in vivo and in vitro were blocked by NS1 - immune polyclonal mouse serum or monoclonal antibodies to NS1, and immunization of mice with NS1 from DENV1 to DENV4 protected against lethal DENV2 challenge.
According to the authors, this is a significant advantage over other animal - derived polyclonal antibody approaches to the treatment of disease, where the antibody must be treated with enzymes to «despeciate» the antibodies to prevent a condition known as serum sickness.
After incubation with 3 % hydrogen peroxide for 10 minutes and 0.2 % horse serum for 20 minutes, a 1:200 diluted rabbit anti-DDX3 polyclonal antibody was applied for 1 hour at room temperature and followed by incubation with biotinylated linker and streptavidin - horseradish peroxidase (LSAB2 system - HRP, DAKO Cytomation, Carpinteria, CA) for 30 and 10 minutes, respectively.
Serologic testing at CDC utilized a Western blot (WB) assay that showed excellent sensitivity to MuLV and XMRV polyclonal or monoclonal antibodies, and no reactivity on sera from 121 US blood donors or 26 HTLV - and HIV - infected sera.
We are grateful to Dr. Robert Silverman at the Cleveland Clinic for the VP62 XMRV plasmid, Dr. Ila Singh at the University of Utah for the rabbit anti-XMRV polyclonal sera, and Dr. Sandra Ruscetti at the National Cancer Institute for the rat anti-SFFV (7C10) monoclonal antibody.
Their study — the first to examine Nab specificities in a cohort of superinfected individuals, suggests that exposure to diverse HIV subtypes following superinfection may drive a broad and potent Nab response that might be mediated at least in part by polyclonal antibodies targeting multiple different epitopes, or antigenic targets found on the virus.
An Alexa Fluor ® 555 - conjugated goat anti-mouse polyclonal (1/500) was used as the secondary antibody.
A Th2 profile with increase in the numbers of CD4 + and CD8 + T cells secreting IL - 4 following polyclonal stimulation, including in resting cells, was demonstrated in CFS patients [13].
Membranes were then incubated with the same blocking solution containing rabbit polyclonal primary antibodies against HIF - 2α (1:500, ab199).
«The finding that metastases are frequently polyclonal and that subsequent cellular behavior is site - dependent also gives us insight into the origins and evolution of clonal diversity in metastatic disease.»
Slava Stamova (Rieber, TUD)-- «Polyclonal tumor - specific activation of cytotoxic effector cells by bispecific antibody constructs» (2009)
Among other applications, its innovative technological platform enables identifying and analyzing the genetic diversity of B cells to convert them into polyclonal recombinant antibodies (biotherapeutics) that improve outcomes for patients with severe diseases.
Is there any difference between the specificity of the Rabbit Anti-CD3 Monoclonal Antibody, Unconjugated, Clone SP7 cat # ab16669 and Rabbit Anti-CD3 epsilon Polyclonal Antibody, Unconjugated cat #ab49943?
We validated the purified, massively polyclonal protein product with in vitro and mouse models.
The affinity purified polyclonal antibodies produced by these species have binding properties that are similar to those derived from the more familiar host species.
Sustained high - level polyclonal hematopoietic marking and transgene expression 4 years after autologous transplantation of rhesus macaques with SIV lentiviral vector - transduced CD34 + cells.
For samples containing TRB clonal populations, the yield is up to four prominent amplified products of the same length and sequence that are detected with significant frequency of occurrence, within a diminished polyclonal background amplified at a lower frequency.
The performance of Western blot and the generation of rabbit polyclonal anti-RIG-G sera were described in our previous work (2).
Titration of polyclonal MuLV goat antisera in Western blot (WB) assay.
Until panels of well - characterized clinical specimens become available, assay validation will be limited to reagents generated experimentally, such as polyclonal and monoclonal antibodies, XMRV plasmids, and XMRV - infected cells.
Secondary was a Goat anti-rabbit polyclonal Texas Red ® conjugate at 1/200.
Sensitivity of the assay was estimated using two fold serial dilutions of the MuLV, XMRV, and SFFV polyclonal and monoclonal antibodies.
Secondary used was a goat anti-rabbit polyclonal biotin conjugate at 1/300.