Sentences with phrase «promoter region»

We searched for over-representation of DNA binding motifs in promoter regions of the 197 differentially expressed genes presented in Supplementary Table 3 using Pscan (28) and the JASPAR database (29).

While they spotted mutations in promoter regions in the genomes of pancreatic cancer cells, «these mutations weren't near any of the genes that prior research had already told us were typically mutated in pancreatic cancer,» says Michael Feigin, Ph.D., a postdoctoral researcher who led the work in the lab of CSHL Professor David Tuveson, M.D., Ph.D..

Early life adversity and serotonin transporter gene variation interact to affect DNA methylation of the corticotropin - releasing factor gene promoter region in the adult rat brain

Association between the recombinant human serotonin transporter linked promoter region polymorphism and behavior in rhesus macaques during a separation paradigm

While SIRT1 and 2 inhibit NF - kB through posttranslational modification, SIRT6 neutralizes it by decreasing the accessibility of p65 subunit on promoter region of NF - kB target genes 32.

Maybe if virus - related cancers have methylated DNA promoter regions of immune - related genes, the answer to increasing the effectiveness of immune - based therapies against cancer is to demethylate these genes.

Socioeconomic status mediates the genetic contribution of the dopamine receptor D4 and serotonin transporter linked promoter region repeat polymorphisms to externalization in preadolescence

Family - based association study of the serotonin transporter promoter region polymorphism (5 - HTTLPR) in attention deficit hyperactivity disorder

Dwarki and Jaime Escobedo improved the AAV's ability to insert genes into chromosomes by adding a gene promoter region from cytomegalovirus, known to be active in the target for their gene therapy, muscle cells.

Second, the TERT promoter region was evaluated.

«Using this system, we can visualize the expression of a fluorescent protein and monitor the transcriptional activity from the E-cadherin promoter region as fluorescent intensity, allowing the screening of EMT - suppressive miRNAs.»

These results suggest that divergent transcription over short distances is common for active promoters and may help promoter regions maintain a state poised for subsequent regulation.

«You don't want to just turn down methylation globally, which would result in over-activation of all genes in the cell, but demethylating some of these gene promoter regions selectively could revive an immune system muted by cancer - causing viruses,» Kuss - Duerkop says.

These sequences in our DNA are often situated far away from the actual genes they regulate, in comparison to so called promoter regions, which traditionally have been considered to control most of the gene activity.

During inflammation reduced levels of NAD + do not impaired the hyperacetylation of NF - κB which in turn through promoter region of target genes activate pro-inflammatory pathway and senescence.

To construct the p21waf1 / cip1 internal promoter region (nucleotide − 127 to − 63) deleted reporter plasmid (− 2,325 / +8) Δ (− 127 / − 63) p21 - Luc, the p21 - Luc construct was digested with ApaI, blunted, and then self - ligated.

Moreover, in this work, the DDX3 - responsive region is delineated to 60 bp (between − 123 and − 63) of the p21waf1 / cip1 proximal promoter region (see Fig. 3B), and the integrity of multiple tandem Sp1 sites in this region is required for the activation induced by DDX3 (see Fig. 3C).

Brd4 forms complexes with chromatin via two tandem bromodomains (BD1 and BD2) that bind to acetylated lysine residues in histones and Brd4 association with acetylated chromatin is believed to regulate the recruitment of elongation factor b and additional transcription factors to specific promoter regions.

As far as results go, Dr. Shendure showed a histogram: on the X-axis was each base of the core promoter region that they evaluated, and on the Y - axis, the effect of mutating that position on transcription.

Primers specific for the hygromycin resistance gene were used alongside control primers to sites in the Hex promoter region.

Identification of a novel sequence element in the common promoter region of human collagen type IV genes, involved in the regulation of divergent transcription

In rats, variations in maternal care in the first week of life are associated with alterations in DNA methylation and H3K9 acetylation of the NR3C1 promoter region, and gene expression of the GR17 splice variant of the NR3C1 gene in the hippocampus of adult offspring [6].

We tested the hypothesis that a repeat polymorphism in the rhesus macaque MAOA gene promoter region influences aggressive behavior in male subjects.

Mesh terms: Aggression Analysis of Variance Animals Behavior, Animal Genetic Variation Macaca mulatta Male Maternal Deprivation Monoamine Oxidase Polymorphism, Genetic Promoter Regions, Genetic Random Allocation Social Environment

They studied the contributions of various genetic control mechanisms, including epigenetic changes such as methyl tags on promoter regions in the DNA and histone modifications, to understand how these different levels of regulation interacted with genetic differences to change the expression of genes, immune function and, ultimately, human disease.

In this case, viruses cause methylation of parts of the genome known as DNA promoter regions.

The TERT promoter region was not included in the multiplex PCR because of its high GC content.

This investigation examined the extent to which polymorphisms of the serotonin transporter linked promoter region (5 - HTTLPR) and the dopamine receptor D4 (DRD4) genes differentially influenced the development of attachment security and disorganization in maltreated and nonmaltreated infants at age 13 months, and the extent to which the efficacy of preventive interventions to promote attachment security were influenced by genetic variation.

Early life adversity and serotonin transporter gene variation affect DNA methylation of the corticotropin - releasing factor gene promoter region in the adult rat brain

Higher 5 - HTT CpG methylation, but not sequence variation in the serotonin transporter promoter region, exacerbates the effects of early life stress on behavioral stress reactivity.

Since their structural research had shown the ZMYND11 / H3.3 combination localized in a gene's DNA rather than its promoter region, the team hypothesized that it fine - tunes gene expression during elongation rather than acting as an on - off switch in the gene's promoter region.

Gene activation begins when a transcription factor connects with the gene's promoter region.

A genetic variation, or allele, found in the promoter region of the serotonin transporter was found to affect this brain loss.

This molecular complex then binds to the promoter region of a gene called Bmi1, an event that increases the expression of the gene.

In other words, by methylating DNA promoter regions, viruses can turn off genes.

Think of these promoter regions like on - off switches for next - door genes — when a promoter region is methylated, the switch is turned off and the gene it controls does not get read and expressed.

The researchers found that MYC may affect circadian rhythm and metabolism by promiscuously binding to promoter regions in key genes for maintaining these daily cycles.

Sparks used a modified version of a technique called a yeast one - hybrid assay to identify more than 20 root proteins that would likely bind to the promoter region of the Short - root gene to control its activity.

The new findings should encourage researchers to look for molecules that either block the sticky ends from attaching or block the protein from binding to the promoter region, says Gamarnik.

«We found that the Tcl1 gene was upregulated by KLF4 binding to its enhancer and promoter regions,» study co-first author Ken Nishimura says.

A functional polymorphism in the promoter region of the human serotonin transporter gene (SLC6A4) has been associated with several dimensions of neuroticism and psychopathology, especially anxiety traits, but the predictive value of this genotype against these complex behaviors has been inconsistent.

The aim of the game is to improve the sequence alignment of promoter regions — which control when a gene is transcribed — of 521 disease - associated genes from 44 vertebrate species.

In particular, they examined different forms of the promoter region of the vasopressin V1a receptor gene (AVPRIA), known as DupA and DupB.

They then asked why biglycan expression was increased in the endothelial cells, and found that the promoter region of the gene was de-methylated, meaning the gene was switched on.

In both cases, the scientists didn't touch the promoter region, which controls how much protein is made.