Did the students have a list
of safe sites to use for their research, appropriate for their grade level?
To our knowledge, there is no published experimental evidence for
safe sites on bees.
It also should be verified whether there are differences in grooming activity and pollen deposition
in safe sites of bees searching for nectar as well as in those of bees actively collecting pollen.
Safe sites also work best in places where drug use is centralized, such as in specific urban neighborhoods rather than rural areas.
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Contrary, in A. mellifera, the thorax and
abdomen safe sites were similar in size (p = 0.9 according to Tukey post-hoc test, Fig 2A).
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With the combination of experimental contamination of bees with pollen and subsequent grooming by the bees we have been able to precisely
characterise safe sites on the bees» body.
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The risk of overdose or arrest does not stop addicts, so
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We look at this as being a real fight, a real battle for preservation, so that the butterflies will have a
wintering safe site.»
The larger the congruence between the area contaminated with pollen by the pollen - sacs or that has been contacted by the stigma and the
respective safe site of the corresponding pollinator, the lower is the probability of losing pollen according to the bees» grooming activity.
This method allows the determination of areas on the bees» body that are not groomed by the legs as well as the detailed documentation of the
potential safe sites» positions and sizes, and the amount of pollen deposited.
There was no significant difference between the number of pollen grains of the head and
thorax safe sites (caput: p > 0.2, Z = -1.1, thorax: p > 0.4, Z = -0.9)(Fig 2B, S2 Table).
Therefore, to better
identify safe sites and their importance for pollination, it is also necessary to develop experimental settings.
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Safe site area (A): B. terrestris: caput n = 19, thorax n = 32, abdomen n = 17, A. mellifera: caput n = 14, thorax n = 20, abdomen n = 8.