Sentences with word «transcriptional»

Mechanistic study of transcriptional regulation of stress - responsive Sestrin genes by tumor suppressor p53

It aims to elucidate the roles of transcriptional regulators and chromatin structure in defining stem cell identity.

Luciferase activity indicates transcriptional activity of the p53RE, and β - galactosidase activity of RSV - lacZ was used to normalize the luciferase activity.

Consequently, we found that si - IRF - 1 could significantly reduce IFNα - induced luciferase activity in STAT1 - transfected U3A cells (Fig. 4A), showing that IRF - 1 was indeed involved in transcriptional activation of RIG - G.

As cells divide and differentiate, changes in transcriptional profile occur, leading to considerable phenotypical diversities forming different subpopulation of cells and tissues in living organisms.

«Our findings show that population differences in transcriptional responses to immune activation are widespread, and that they are mainly accounted for by genetic variants that differ in their frequencies between human populations,» said Lluis Quintana - Murci of Institut Pasteur and CNRS in Paris, France, who led one of the two studies.

In the absence of Wnt signaling, we found that Tcf factors associate with proteins of the Groucho family of transcriptional repressors to repress target gene transcription.

In this study, the team tested the counter-intuitive notion that compounds that increase noise in gene expression could work together with transcriptional activators to increase overall levels of HIV reactivation.

Moreover, each subtype of glioma stem cells is driven by distinct transcriptional programs for growth and treatment resistance, and these different cell populations correspond to well - known morphological differences within the GBM itself.

HIPK2 - Mediated Transcriptional Control of NMDA Receptor Subunit Expression Regulates Neuronal Survival and Cell Death

Interestingly, the most commonly mutated set of genes were those involved in epigenetic modification (25 % of tumors) followed by transcriptional regulators and MAP kinase - associated genes (12 - 15 %).

In parallel, the mRNA expression of key transcriptional factors for Th1 cells (T - bet), Th17 cells (Rorc), and Tregs (Foxp3), as well as Ifng and Il17a, also were decreased in a dose - dependent manner (Supplemental Fig. 4D).

Research by Sharon Dent and colleagues revealed for the first time that histone modifications - instead of being an end - point for transcriptional regulation - can actually relay information to non-histone proteins, and that the role of chromatin in these events is not limited to DNA - templated events like gene activation.

Next up was Didier Trono who talked about how transposable elements and their epigenetic control mechanisms are key regulators of transcriptional networks in PSCs.

Elias KM, Emori MM, Westerling T, Long H, Budina - Kolomets A, Li F, MacDuffie E, Davis MR, Holman A, Lawney B, Freedman ML, Quackenbush J, Brown M, Drapkin R.: Epigenetic remodeling regulates transcriptional changes between ovarian cancer and benign precursors.

The study looked at transcriptional profiles of 247 tissue samples from six humans, five chimps, and five macaques.

She and her colleagues knew that chromatin regulators — which control how genetic material gains access to a cell's transcriptional machinery — were key to treating the social deficits in ASD, but the challenge was to know how to affect key risk factors at once.

Rather these states may represent distinct reversible transcriptional signatures affecting key lineage regulators.

Among the genes they validated as transcriptional targets of RORA is the gene for aromatase, an enzyme that converts male to female hormones.

To demonstrate the capabilities of SpDamID in the current study, the authors analyzed Notch - mediated transcriptional activation in mouse kidney progenitor cells (mK4 cells).

Another area of research in the lab concerns the mechanisms by which small double stranded RNAs can trigger transcriptional gene silencing and activation by modulating the histone code.

Based on transcriptional mechanisms of the midbrain dopamine neuronal system, Dr. Kim's lab focuses on developing novel mechanism - based and neuroprotective therapeutics for PD and other neurodegenerative and neuropsychiatric disorders.

For instance, the mono - allelic expression of imprinted genes shows that two alleles exposed to the same transcription factors can have opposite transcriptional states.

The greatest significance was detected at rs227727, about 105 kb downstream of the NOG transcriptional start site.

They found that L2 - deficient eggs were unable to undergo global transcriptional silencing, a process that normally happens in the final stages of egg growth.

Instead, they are organized into a three - dimensional genome structure that participates in various nuclear processes such as transcriptional regulation, DNA replication and repair, as well as chromatin domain formation.

Numb is also a known regulator of Notch, a protein implicated in stem cell maintenance, and together, these observations have driven the development of new stem cell research projects, ranging from transcriptional profiling analysis of normal and cancer stem cells, to the in - depth characterisation of a number of critical signalling / endocytic pathways involving Numb / Notch / p53 (2, 3).

She received her Ph.D. in physiology from the University of Western Ontario, focusing on transcriptional regulatory networks in cell differentiation, and completed postdoctoral training at the Institute for Systems Biology in Seattle, Washington and within the Investigative Toxicology Group at Pfizer in Groton, Connecticut.

A novel transcriptional signaling pathway in the interferon system mediated by 2» -5» - oligoadenylate activation of RNase L. Proc.

This table lists putative transcriptional regulatory targets for the central gene represented on this page.

In Class I engagement, Sox9 binds to the genome indirectly via the basal transcriptional complex, regulating transcription of genes for basal cell activities.

For example, we discovered how the protein phosphatase calcineurin and its downstream effector protein NFAT act synergistically with the transcription factor MEF2 at transcriptional enhancer elements of selected genes, such as myoglobin, to medicate adaptive mechanisms by which skeletal myofibers acquire specialized contractile and metabolic properties in response to changing patterns of muscle contraction.

In addition, these agents specifically regulate the induction of a highly specific transcriptional program in brain cells, which may contribute to the limited clinical efficacy of antidepressant drugs in pediatric patients.

The ATPase activity, but not the helicase activity, of DDX3 contributes to DDX3 - mediated transcriptional modulation on the p21waf1 / cip1 promoter.

He utilizes Drosophila genetics, bioinformatic (RNA - Seq, ChIP - Seq), and molecular genetic approaches to identify transcriptional regulators of circadian clocks and longevity.

Dr Mohammed at the Babraham Institute, said: «Our analyses suggest that elevated transcriptional noise at two key points in early development coincides with cell fate decisions.

TERT promotes epithelial proliferation through transcriptional control of a Myc - and Wnt - related developmental program.

Mianserin blocked signals related to the regulation of serotonin and this delayed physiological changes associated with age, including the newly - identified transcriptional drift and degenerative processes that lead to death.