Sentences with phrase «transfection efficiency»
"Transfection efficiency" refers to how well foreign genetic material, such as DNA or RNA, is introduced and incorporated into a target cell. It measures the success rate of delivering and expressing the desired genes in the cells. Full definition
This cell line permits higher transfection efficiencies than primary neurons which facilitated molecular and biochemical analyses [18].
neuronalsignaling.org
Now, researchers at the Department of Environmental and Life Sciences and Electronics - Inspired Interdisciplinary Research Institute (EIIRIS) in the Toyohashi University of Technology have developed a novel gene - transfection method: water - in - oil (W / O) droplet electroporation with sufficient transfection efficiency and cell viability.
The TK - Relina reporter (Promega) was also cotransfected for normalization of transfection efficiency.
To achieve sparse labeling of individual neurons one could reduce the plasmids amounts to reduce transfection efficiency.
The co-delivery of small molecular drugs with nucleic acids can improve gene transfection efficiency, reduce side - effects of these drugs, and achieve the synergistic effect of drug and gene therapy for the more effective treatment of cancer.
Luciferase assays were analyzed based on the ratio of Firefly / Renilla to normalize cell number and transfection efficiency.
Transfection efficiency was around 95 %.
The total STAT2 level was used to control the transfection efficiency.
Ten nanograms pRL - TK vector (Promega) were used as an internal control for normalizing the transfection efficiency.