Sentences with word «vitripennis»

In order to answer this question, a team of researchers in Germany turned to the Nasonia vitripennis wasp, a species famous for its propensity to lay its parasitic eggs on doomed fly pupae.

Male wing size increases by 45 % through cell size and cell number changes when the ws1 allele from N. giraulti is backcrossed into a N. vitripennis genetic background.

This suggests that the ability to produce the RS - RR mixture may have started off as a neutral genetic mutation among an earlier lineage of N. vitripennis males.

The mapped markers enabled us to arrange 265 scaffolds of the Nasonia genome assembly 1.0 on the linkage map, representing 63.6 % of the assembled N. vitripennis genome.

Wing measurements were conducted using the inbred N. vitripennis strain AsymC and inbred N. giraulti strain R16A; these data are also reported in [15].

If N. vitripennis females ultimately come to prefer the RS - RR pheromone blend, they must eventually start associating it with males of their own species and evolve a way to recognize it.

Scientists and government officials sought his help in French Polynesia, where islands were overrun with the glassy - winged sharpshooter, or Homalodisca vitripennis.

Bordenstein and Brucker wanted to know whether these dissimilar microbiomes were the reason the N. vitripennis hybrids didn't make it.

We aligned our RNA - seq reads to the N. vitripennis reference genome version 1.0 [15], available from NasoniaBase.

Citation: Sackton TB, Werren JH, Clark AG (2013) Characterizing the Infection - Induced Transcriptome of Nasonia vitripennis Reveals a Preponderance of Taxonomically - Restricted Immune Genes.

Gene expression experiments were conducted with the N. vitripennis AsymCX strain used for genome sequencing [10], which was derived from AsymC by multiple generations of sib - mating.

We are grateful to J. Romero - Severson for developing the N. vitripennis BAC library; Stephen Richards and the Baylor Human Genome Sequencing center for sequencing of BAC clones as part of the Nasonia genome project; Rhitoban Raychoudhury, Jen Traggis, Laramy Enders, and Adityarup Chakravorty for assistance with laboratory work; Heinrich Jasper for advice on qPCR; and Claude Desplan for comments on the manuscript.

However, quantitative RT - PCR reveals an estimated 2.4 X higher level of dsxM transcript in developing prepupal male wings of ws1v relative to ws1g in the same vitripennis genetic background (Figure 4; U-test, p = 0.04, n = 7 biological replicates).

Our data show an overall negative trend between the recombination rate and both the amino acid distance (dA) and the ratio of non-synonymous to synonymous substitutions per site (ω) when comparing N. vitripennis exon sequences with those of N. giraulti and N. longicornis.

Insights into the venom composition of the ectoparasitoid wasp Nasonia vitripennis from bioinformatic and proteomic studies..

When the scientists analyzed the N. vitripennis male sex pheromone, they found it contained two important chemicals, which they call RS and RR.

N. vitripennis females preferred a blend of RS and RR, but a whiff of RS alone was enough to get them in the mood, the researchers report online today in Nature.

We found that CI resulted from delayed nuclear envelope breakdown of the male pronucleus in Nasonia vitripennis.

To positional clone this major sex - specific wing QTL and to more precisely describe its phenotypic effects we (a) reduced the size of the introgressed sequence flanking the ws1 locus to a 40kb segment (see fine - scale mapping and cloning below) and (b) backcrossed the introgressed ws1g segment into a standard N. vitripennis strain (AsymCX) genetic background for > 10 generations.

Assuming that the inferred Nasonia linkage map is saturated (i.e., the mapping of additional scaffolds would not lead to its expansion) and that the physical size estimate of the N. vitripennis genome (312 Mb) is accurate, we estimated a genome wide recombination rate of 1.4 cM / Mb.

N. giraulti male forewings are 2.16 fold larger in area than N. vitripennis male forewings, although female wings of both species are large and more similar in size (Figure 1; Table 1; [13], [15]-RRB-.

To test their idea, they raised the hybrid offspring of N. vitripennis and each of the other wasp species in a germ - free environment, eliminating any signs of gut bacteria that might be toxic to the wasps.

Bordenstein believes that this mismatch can drive a wedge between two groups of organisms until their offspring can no longer survive, ultimately resulting in a separate, new species akin to N. vitripennis.

But when either wasp tried to mate with the related Nasonia vitripennis, which has different gut microbes, those hybrid offspring died.

The third species, N. vitripennis, is less similar.

In a paper in the 10 May issue of Science, geneticists William Sullivan and Uyen Tram at the University of California, Santa Cruz, offer the first good glimpse of how Wolbachia do this, gleaned from studying the wasp Nasonia vitripennis.

We also produce a new homology - based immune catalog in N. vitripennis, and show that most infection - induced genes can not be assigned an immune function from homology alone, suggesting the potential for substantial novel immune components in less well - studied systems.

Second, we extracted reciprocal best blastp hits (RBH) between N. vitripennis and D. melanogaster.

As a complement and extension of our new homology - based immune annotation of the OGSv2 gene models, we characterized the infection - induced transcriptome in N. vitripennis to identify genes up - regulated by infection.

Here, we use RNA - seq to compare the uninfected and infection - induced transcriptome in the parasitoid wasp Nasonia vitripennis to identify genes regulated by infection.

Together, these form the most complete annotation of immune - related genes in N. vitripennis to date, and represent one of the first genomic - scale annotations of novel immune - induced transcripts in Hymenoptera.

Eradicating the full complement of the wasps» microbes allowed hybrids of N. vitripennis and its two distant relatives to survive.

HGD is divided into three main divisions: BeeBase, which hosts bee genomes and the Bee Pests and Pathogens resource; NasoniaBase, which hosts the genome of the Jewel wasp (Nasonia vitripennis); and the Ant Genomes Portal, which hosts the genomes of several ant species.

Normally, when members of two related species of parasitic wasps in the genus Nasonia, N. giraulti and N. longicornis, mate with their more distant relative N. vitripennis, the hybrid offspring die.

This strain contains ∼ 40Kb of introgressed giraulti DNA containing ws1g in a vitripennis genetic background.