The tissue was paraformaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed before the tissue was blocked and incubated with the antibody for 1 hour. (abcam.com)
A heat mediated antigen retrieval step was performed with Tris - EDTA buffer (pH9). (ptglab.com)
Slides were incubated in antigen retrieval buffer (DAKO, Carpinteria, CA,) supplemented with 0.3 % Tween 20 and boiled at 100 ˚C for 10 minutes. (jcancer.org)