Briefly, retinal samples were homogenized at 4 °C in buffer containing 10 mM Tris (pH 7.4), 1 mM EDTA, 0.15 M sodium chloride, 0.5 % NP - 40, with protease inhibitors (20 µg / ml aprotinin, 5 µg / ml leupeptin, and 1 mM phenylmethane sulfonyl fluoride - PMSF). (molvis.org)
Cells were lysed with TN1 lysis buffer (50 mM Tris, 125 mM NaCl, 1 % Triton X-100, 10 mM EDTA, 10 mM sodium fluoride and 10 mM sodium pyrophosphate) supplemented with protease inhibitor cocktail (1.2 mM AEBSF, 0.46 μM aprotinin, 14 μM bestatin, 12.3 μM E-64, 112 μM leupeptin, 1.16 μM pepstatin)(Amresco; Solon, OH)-RRB- and 1 mM sodium orthovanadate (Enzo Life Sciences; Farmingdale, NY). (journals.plos.org)
Kemppainen RJ, Clark TP, Peterson ME: Aprotinin preserves immunoreactive adrenocorticotropin in canine plasma. (animalendocrine.com)