The MCF10A mammary epithelial cells were cultured in DMEM - F12 medium supplemented with 5 % horse serum, 20 ng / ml EGF, 10 μg / ml insulin, 0.5 μg / ml hydrocortisone, 100 ng / ml of cholera toxin, and penicillin / streptomycin. (elifesciences.org)
The protein cholera toxin B, or CTB, a non-toxic carrier currently approved for use in humans by the Food and Drug Administration, is used in this study to traverse the blood - brain barrier. (sciencedaily.com)
Potentiation by cholera toxin of bradykinin - induced inositol phosphate production in the osteoblast - like cell line MC3T3 - E1 (biochemj.org)