Following biotinylated secondary antibody incubation and avidin — biotin complex treatment, immunopositive signals were visualized by diaminobenzidine staining. (biochemj.org)
Immunostaining was detected with diaminobenzidine (Sigma - Aldrich). (eneuro.org)
Stainings were developed with 0.06 % 3,3 ′ - diaminobenzidine (Sigma - Aldrich, USA) and 0.01 % hydrogen peroxide (Sigma - Aldrich, USA) in PBS and then mounted on subbed slides. (actaneurocomms.biomedcentral.com)