The best media available is made from fetal calf serum or horse serum, and you know, that's sort of a nonstarter. (scientificamerican.com)
A team of developmental biologists led by Hans Schöler and Karen Hübner at the University of Pennsylvania placed densely packed clusters of stem cells from mouse embryos in a petri dish, using fetal calf serum as a growth medium and adding a gene protein that turns green when germ cells form. (discovermagazine.com)
All staining was done at room temperature in FACS Wash Buffer (1 mM EDTA, 2.5 % fetal calf serum in PBS) and all antibodies were from BD Biosciences unless otherwise noted. (journals.plos.org)