Beginning 72 hours post-stimulation, cells were counted every 48 hours using trypan blue dye exclusion on an automated hemocytometer (Countess, Invitrogen) and split to 0.8 × 106 with fresh media containing 100 U / ml IL - 2. (journals.plos.org)
The pellet was resuspended (1 % bovine serum albumin in PBS), and total inflammatory cell numbers were determined via an improved Neubauer hemocytometer (ProSciTech, Thuringowa, Qld, Australia). (jpet.aspetjournals.org)
Following gesicle treatment, CD81 - negative hiPS cells sorted via flow cytometry were counted using a hemocytometer. (takarabio.com)