Synaptophysin in (f) by immunoblot by in brain homogenates; for regional effects on synaptophysin and MAP2, analyzed in serial sections by immunohistochemistry, see Fig. 8 in [22]-RRB-. (sens.org)
HuH - 7 cell extracts (lane 3) or FLAG - Sp1 - containing cell extracts (lane 4; 1.5 mg each) were immunoprecipitated with anti-FLAG antibody — conjugated beads and the immunoprecipitates were analyzed by SDS - PAGE followed by immunoblotting with anti-FLAG antibody or anti-DDX3 antibody. (cancerres.aacrjournals.org)
For immunoblot analysis, 50 μg protein from each total cell lysate was electrophoresed through 10 % SDS - polyacrylamide gels, then transferred to PVDF membranes (Bio-Rad). (jci.org)