Protein was extracted into 200 μl of Luciferase Cell Culture Lysis Reagent (Promega), and 40 μl of protein extract was used for luciferase assays. (journals.plos.org)
The cells were cotransfected with the reporter gene constructs and related expression plasmids as indicated by using SuperFect (Qiagen) followed by transcriptional activity assay using Dual - Luciferase Assay System (Promega) according to the manufacturer's instruction. (cancerres.aacrjournals.org)
His team looked at an experimental drug called PTC124, which the firefly luciferase assay had identified as a promising candidate for treating stop - sign forms of cystic fibrosis and which is now being tested in clinical trials. (sciencemag.org)