Pluripotent cells can be created by introducing transcription factor genes into mouse embryonic fibroblasts on a plasmid that does not integrate into the genome. (science.sciencemag.org)
For this study, Guttridge, first author David J. Wang, who developed many of the study's concepts, and their colleagues monitored NF - kB activity during tumor development using mouse embryonic fibroblasts and two mouse models. (sciencedaily.com)
Repeated transfection of two expression plasmids, one containing the complementary DNAs (cDNAs) of Oct3 / 4, Sox2, and Klf4 and the other containing the c - Myc cDNA, into mouse embryonic fibroblasts resulted in iPS cells without evidence of plasmid integration, which produced teratomas when transplanted into mice and contributed to adult chimeras. (science.sciencemag.org)